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Quantitative PCR detection of Batrachochytrium dendrobatidis DNA from sediments and water: Diseases of Aquatic Organisms

Authors: Julie A Kirshtein; Chauncey W Anderson; J S Wood; J E Longcore; M A Voytek
Contribution Number: 268

http://www.int-res.com/articles/dao_oa/d077p011.pdf

Abstract/Summary

The fungal pathogen Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a disease implicated in amphibian declines on 5 continents. We developed filtering and PCR based quantiative methods to detect Bd in water and sediments. Our methods provide a new tool to investigate critical aspects of Bd in the environment.

Publication details
Published Date: 2007
Outlet/Publisher: Diseases of Aquatic Organisms 77: 11-15
Media Format: .PDF
ARMI Organizational Units:
Pacific Northwest - Water
National Headquarters
Topics:
Disease
Stressors
Water
Place Names:
Glacier National Park
Rocky Mountain National Park
United States
Keywords:
amphibians
Bd
chytrid fungus
Chytridiomycosis
detection
disease
filter
methods
monitoring
pathogen
PCR
primers
qPCR
stressors
water
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