Quantitative PCR detection of Batrachochytrium dendrobatidis DNA from sediments and water: Diseases of Aquatic Organisms

Authors: Julie A Kirshtein; Chauncey W Anderson; J S Wood; J E Longcore; M A Voytek

Contribution Number: 268

http://www.int-res.com/articles/dao_oa/d077p011.pdf

Abstract/Summary

The fungal pathogen Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a disease implicated in amphibian declines on 5 continents. We developed filtering and PCR based quantiative methods to detect Bd in water and sediments. Our methods provide a new tool to investigate critical aspects of Bd in the environment.

Publication details

Published Date:	2007
Outlet/Publisher:	Diseases of Aquatic Organisms 77: 11-15
Media Format:	.PDF

ARMI Organizational Units:

Pacific Northwest - Water
National Headquarters

Topics:

Disease; Stressors; Water

Place Names:

Glacier National Park; Rocky Mountain National Park; United States

Keywords:

amphibians; Bd; chytrid fungus; Chytridiomycosis; detection; disease; filter; methods; monitoring; pathogen; PCR; primers; qPCR; stressors; water

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